Identification of the antigenic proteins of Cowdria ruminantium.
نویسندگان
چکیده
Immunoblotting of Cowdria ruminantium proteins with sheep or bovine antiserum identified 2 antigenically conserved proteins, one being an immunodominant 31 kDa and the other a minor 27 kDa protein. These proteins are present in the electrophoretic profiles of the Welgevonden, Ball 3 and Kwanyanga stocks and are recognized by sheep antiserum to the Welgevonden, Ball 3, Kwanyanga, Mali, Comoro, Breed, Germishuys, Kümm and Mara stocks and by bovine antiserum to the Welgevonden stock of C. ruminantium. The stocks did not reveal identical or unique antigenic properties which could explain differences in pathogenicity and cross-immunity observed amongst the various stocks of C. ruminantium.
منابع مشابه
Identification of an immunodominant antigenically conserved 32-kilodalton protein from Cowdria ruminantium.
Western blotting (immunoblotting) of Cowdria ruminantium antigens with goat or mouse antiserum identified a periodate-resistant, proteinase K-sensitive immunodominant antigen of 32,000 daltons. This protein, designated Cr32, could be demonstrated in goat choroid plexus infected with one of two different Cowdria stocks. Antisera against nine different Cowdria stocks from Africa and the Caribbean...
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An immunohistochemical staining technique in which a monospecific serum was used against the major antigenic protein -1 (MAP-1) of Cowdria ruminantium, was evaluated for the detection of C. ruminantium in formalin-fixed tissues of experimentally infected mice and field cases of heartwater in sheep, cattle and goats. Mice were infected with the mouse-pathogenic stocks: Mara, Kwanyanga, Welgevond...
متن کاملIdentification of drugs effective against Cowdria ruminantium using a mouse screening system.
Heartwater is a tick-transmitted rickettsial infection of ruminants, caused by Cowdria ruminantium. The tetracyclines are the only compounds available for therapy of the disease. A screen, using mice infected with C. ruminantium, was developed and used to identify new compounds with potential for the control of heartwater. A series of di-4-methyl-thiosemicarbazones was shown to be highly active...
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• Heartwater can cause high mortality amongst cattle, sheep and goats. It is caused by the tick-borne organism Cowdria ruminantium. • The disease has a major impact upon the development of livestock owning communities especially those in small-holder dairy schemes. • Existing vaccines against heartwater, a tickborne disease which is a major constraint to small ruminant production in sub-Saharan...
متن کاملIdentification of Babesia bovis and Cowdria ruminantium on the island of Unguja, Zanzibar.
Babesia bovis and Cowdria ruminantium were identified for the first time in cattle on Unguja Island, Zanzibar. B bovis is common and widespread, although clinical disease had not been diagnosed previously. The vector of heartwater, Amblyomma variegatum, is found throughout Unguja but C ruminantium appears to be more localised in distribution than B bovis.
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ورودعنوان ژورنال:
- The Onderstepoort journal of veterinary research
دوره 57 4 شماره
صفحات -
تاریخ انتشار 1990